Determination of Nucleotide Sequence of a Region Producing Multiple Polygalacturonases from Erwinia carotovora subsp. carotovora EC1.
نویسندگان
چکیده
منابع مشابه
Erwinia carotovora subsp. carotovora extracellular protease: characterization and nucleotide sequence of the gene.
The prt1 gene encoding extracellular protease from Erwinia carotovora subsp. carotovora EC14 in cosmid pCA7 was subcloned to create plasmid pSK1. The partial nucleotide sequence of the insert in pSK1 (1,878 bp) revealed a 1,041-bp open reading frame (ORF1) that correlated with protease activity in deletion mutants. ORF1 encodes a polypeptide of 347 amino acids with a calculated molecular mass o...
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Erwinia carotovora subsp. carotovora, a Gram-negative phytopathogenic bacterium, secretes an extracellular metalloprotease, PrtW. Previous results demonstrated that protease activity is necessary for the normal progression of disease symptoms caused by this bacterium. The present study revealed that the prtW gene constitutes an independent transcriptional unit. It is demonstrated that introduct...
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The polygalacturonase, clearly differentiated from polygalacturonic acid trans-eliminase, produced in the culture fluid of Erwinia carotovora was purified 56-fold by means of acetone precipitation, acetate butfer extraction, and two successive treatments by chromatography on carboxymethyl cellulose. The purified enzyme is specific for nonmethoxylated polygalacturonic acid. It shows an optimum p...
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ژورنال
عنوان ژورنال: Japanese Journal of Phytopathology
سال: 1994
ISSN: 1882-0484,0031-9473
DOI: 10.3186/jjphytopath.60.208